Biology 9700 Practical Notes (2026)

An eyepiece graticule has arbitrary units. You must calibrate it against a stage micrometer (which has a known scale, typically divisions) for every magnification change.

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The factor you deliberately alter (e.g., pH levels, substrate concentrations).

Acid hydrolyzes glycosidic bonds; alkali neutralizes it before adding Benedict's. Iodine in KI solution Blue-Black Yellow-Brown Completed at room temperature. Proteins Biuret Reagent ( NaOHcap N a cap O cap H CuSO4cap C u cap S cap O sub 4 Purple / Violet Remains Blue Completed at room temperature. Lipids Ethanol Emulsion Test Milky-White Emulsion Clear / Colorless Dissolve sample in ethanol first, then pour into water. 3. Enzyme Kinetics and Variables biology 9700 practical notes

Microscopy questions are a staple of the 9700 practical exam. You must know how to calibrate a microscope and calculate actual specimen sizes. Graticule Calibration

Tonight, she opened a fresh notebook and wrote at the top:

Here’s a short, engaging story based on the phrase An eyepiece graticule has arbitrary units

Draw only the tissue layers (e.g., epidermis, xylem, phloem). No shading or sketching allowed. . Ensure units are converted to micrometers ( ) before calculating. 🧪 Core Skill 2: Biochemical Testing

Acing the isn’t just about knowing your theory; it’s about mastering the "art of the lab." Whether you are facing a titration, a microscopy slide, or a complex enzyme rate experiment, your success depends on precision, observation, and following specific CIE conventions.

A parallel setup where the independent variable is omitted or inactivated (e.g., substituting an active enzyme with boiled, denatured enzyme or distilled water). This proves that the observed change is caused solely by the independent variable. 4. High-Yield Exam Day Blueprint Lipids Ethanol Emulsion Test Milky-White Emulsion Clear /

Write a clear, logical sequence of actions so another scientist could replicate your experiment exactly. Include the specific volumes and apparatus used.

You will usually get a plant stem (T.S.) or an animal tissue.

Print this box and memorize it 24 hours before your exam.

Explicitly state the independent variable (with 5 chosen concentrations), the dependent variable (how and when it is measured), and at least three critical variables to keep constant (with specific methods to control them).

) that the difference is due to chance is less than 0.05. Reject the null hypothesis ( H0cap H sub 0